Fig 1: The effect of restoring miR-369 levels on tau hyperphosphorylation and upregulation of Fyn and SRPK2 in the cerebral cortex of miR-369KO/AD mice (10 mice/group, male:female = 1:1, treatments at 12 months old). (A) Real-time qPCR results indicating the restoration of mature miR-369 expression in the cerebral cortex after delivery of miR-369 in miR-369KO/AD mice. (B–D) Representative western blot images and the relative quantifications of the blots. (E) The results of IHC staining for phosphorylated and total tau proteins and the kinases Fyn and SRPK2, which show that restoring miR-369 significantly reversed the hyperphosphorylation of tau and the upregulation of Fyn and SRPK2 in the cerebral cortex of the mice. *p < 0.05 and **p < 0.01.
Fig 2: Changes in the levels of phosphorylated tau and the kinases Fyn and SRPK2 in miR-369KO/AD mice (12 months old, 10 mice/group, male:female = 1:1). (A–C) The real-time qPCR results indicating the loss of endogenous mature miR-369 expression in the brain regions (CTX, cortex; HIP, hippocampus; and STRM, striatum). (A) No change in MAPT mRNA, which encodes tau protein (B), and the upregulation of mRNA of Fyn and SRPK2 (C) in miR-369KO/AD mice. (D) Representative western blot images. (E–G) The quantitative presentations of the immunoblots. (H) The IHC staining results for tau protein, Fyn, and SRPK2 expression in the cerebral cortex. The results indicate an upregulation of phosphorylated tau protein (at Tyr18 and Ser214) and of Fyn and SRPK2 expression and no change in the total tau levels in miR-369KO/AD mice. *p < 0.05 and **p < 0.01.
Fig 3: Luciferase assay shows the targets of miR-369. (A) Schematic diagram of the target sites of miR-369 on Fyn and SRPK2 mRNAs, which shows that miR-369 has potential complementary sites targeting the mouse Fyn and SRPK2 3'UTRs. (B,C) The results of the luciferase assay showing that miR-369 directly targeted the 3'UTRs of Fyn and SRPK2. All experiments were performed in triplicate. *p < 0.05 and **p < 0.01.
Supplier Page from Abcam for Anti-SRPK2 antibody [EPR16366] - N-terminal